Summer School on Biocatalysis

Juan Pablo Medina, student of Biotechnology at the University Francisco de Vitoria (Madrid) and Leticia Monsalve, student of Chemical Engineering at the Polytechnic University of Madrid, are performing summer practices in our group. The students are doing a fast training on different techniques related with biocatalysis (determination of enzyme activity, immobilization, HPLC to follow the progress of the biotransformations, etc.).

ANQUE·ICCE·BIOTEC 2014 Congress on Chemistry, Chemical Engineering and Biotechnology

The Group had an active participation in the recent ANQUE·ICCE·BIOTEC 2014 Congress on Chemistry, Chemical Engineering and Biotechnology (“Science, the key for a better life”) held in Madrid during 1-4 July 2014. Paloma Santos presented her work on "Enzymatic hydrolysis of chitosan into chitooligosaccharides: analysis of the reaction products by chromatographic techniques" and Francisco Plou  the work performed by Santos Dominguez during his Master Thesis on "Synthesis of prebiotic isomaltooligosaccharides from starch by a combination of amylolytic enzymes”

Conference at the 5th World Congress on Biotechnology

The Applied Biocatalysis Group has participated in the 5th World Congress on Biotechnology held during June 25-27 2014 in Valencia. Francisco Plou gave a conference on "Transformations of carbohydrates into prebiotic oligosaccharides using free or immobilized glycosidic enzymes". The abstract of this paper was published in the Journal of Biotechnology & Biomaterials.

Participation at the XI Carbohydrate Symposium

The Applied Biocatalysis Group has participated in the XI Jornadas de Carbohidratos (XI Carbohydrate Symposium) held in Logroño, Spain, from May 28th to 30th, 2014. Francisco Plou presented an oral communication on "Galactooligosaccharides formation during enzymatic hydrolysis of lactose: Towards prebiotic-enriched milk". The Jornadas de Carbohidratos symposium is the most important national event in Carbohydrate Chemistry, biennially organized under the auspices of the Specialized Group of Carbohydrates of the Spanish Royal Society of Chemistry, RSEQ.  The congress was dedicated to the scientific career of Prof. Manuel Martín-Lomas.

Official picture Applied Biocatalysis Group

We present the new official photograph of the Applied Biocatalysis Group at CSIC, which is currently formed by seven members.

3rd Multistep Enzyme Catalyzed Processes Congress (MECP14)

Our group has participated in the organization of the 3rd Multistep Enzyme Catalyzed Processes Congress (MECP14) held at CSIC headquarters during 7-10 April 2014. More than 140 delegates attended the Symposium. The pdf file of the MECP14 Abstract Book can be downloaded here. After the Congress, the Kick-off meeting of the COST Action CM1303 “SysBiocat” was also held at CSIC headquarters.

Two High Schools from Fuente Palmera and Posadas (Cordoba) visit our laboratory

More than 30 students coming from the High Schools IES Aljandic (Posadas, Cordoba) and IES Colonial (Fuente Palmera, Cordoba) have visited our Biotransformations Laboratory. The students performed a series of experiments in the field of biocatalysis (enzyme kinetics, immobilization, chromatographic analysis, etc.). They also visited other dependencies at the Institute of Catalysis (ICP-CSIC), in particular a laboratory of protein engineering, an anaerobic chamber, a continuous bioreactor and the cold room.

The Biotransformations Laboratory in the Newsletter of Agarose Bead Technologies

Our group appears in the newsletter of the company Agarose Bead Technologies. Our experience in the use of agarose resins to immobilize enzymes is summarized. The complete testimonial can be downloaded here 

Organization of a Course on Analytical and Semipreparative HPLC

In collaboration with the Madrid Official Chemists' College and Association, the members of the Biotransformations Laboratory have organized  for second time (the first was in 2011) an Introductory Course on Analytical and Semipreparative Liquid Chromatography HPLC. Teaching included seminars and practical sessions. The number of participants was 22, coming from different cities in Spain. The practical sessions included the separation of sweeteners in a soft drink by ionic chromatography, the analysis of a mixture of polyphenols by reverse phase HPLC, the separation of the components of a synthetic biodiesel mixture, and the scaling-up of an analytical separation of carbohydrates.

Novel method for neo-erlose synthesis published in Process Biochemistry

We have shown that the beta-fructofuranosidase from X. dendrorhous fructosylates several carbohydrates that contain glucose. In particular, the enzyme produces selectively neo-erlose from maltose. In this way novel hetero-fructooligosaccharides with food and pharmaceutical applications can be obtained. 





"Regioselective synthesis of neo-erlose by the  β-fructofuranosidase from Xanthophyllomyces dendrorhous".  M. Gimeno-Perez, P. Santos-Moriano, L. Fernández-Arrojo, A. Poveda, J. Jiménez-Barbero, A.O. Ballesteros, M. Fernández-Lobato, F.J. Plou. Process Biochemistry, accepted manuscript  available online: 6-JAN-2014, doi:10.1016/j.procbio.2013.12.018

Merry Christmas

The Laboratory of Biotransformations at the CSIC Applied Biocatalysis Group wants to wish all of you a Merry Christmas and a Happy New Year 2014.
Hope, peace and love for you this Christmas!

The Laboratory of Biotransformations participates in Simbiocat II

A Workshop on "Enzymes and industrial biotransformations: symbiosis between the academy and the industry" (SimbioCAT II) was held at the Institute of Catalysis to establish bridges between the research groups and the biotech companies. Our group presented our recent advances in enzyme immobilization and biotransformations involving glycosidic enzymes.

Seminar of Prof. Stephen G. Withers

Professor Stephen G. Withers gave a conference in our institute on “New access to complex glycoconjugates through engineering and evolution of glycosyltransferases and glycosynthases". Prof. Withers, Khorana Professor of Biological Chemistry at the University of British Columbia (Vancouver, Canada), has made substantial contributions to the understanding of mechanisms of glycosidic enzymes, using such information to the development of potential new therapeutics for Influenza, Diabetes and Gaucher disease. 

Participation in the Master of Biotechnology at Autonomous University of Madrid (UAM)

Members of the Biotransformations Laboratory and the Directed Enzyme Evolution Laboratory have participated for sixth year in the Master of Biotechnology at UAM. Teaching included seminars and practical sessions on the biocatalysis field.

Barbara Rodriguez defends her Thesis

Barbara Rodriguez-Colinas has defended her Thesis entitled "Enzymatic synthesis, characterization and prebiotic properties of oligosaccharides employed in infant milk formulas", supervised by Antonio Ballesteros and Francisco J. Plou. She obtained the maximum qualification (Sobresaliente cum laude). Congratulations to her!

Magali Remaud-Simeon visits our Laboratory

Magali Remaud-Simeon, Professor at the Laboratoire Ingénierie des Systèmes Biologiques et des Procédés (INSA-Toulouse), visited our laboratories. She is a renowned
expert on enzyme molecular engineering and catalysis, especially in the field of glycosidic enzymes.

CSIC - IMDEA Energy Workshop

The Group has participated in the CSIC -IMDEA Workshop focused in the R&D technologies in the field of energy  at the Madrid Region. A poster on "The Applied Biocatalysis Group at CSIC: activities in the field of bioenergy" was presented and can be here downloaded

Participation in the LIGNOCEL meeting

Our Group has participated in the LIGNOCEL Meeting held in Pontevedra. This network is focused on biotransformations of lignocellulosic materials, and is formed by 28 research groups and 9 companies

Paper published in Food Chemistry

The formation of galacto-oligosaccharides (GOS) in milk during the treatment with several commercial β-galactosidases (Bacillus circulans, Kluyveromyces lactis and Aspergillus oryzae) was analyzed. Using K. lactis beta-galactosidase, a treated milk with 7.0 g/L GOS −the human milk oligosaccharides (HMOs) concentration is between 5 and 15 g/L−, and with a low content of residual lactose (2.1 g/L, compared with 44-46 g/L in the initial milk sample) was obtained.

Reference: B. Rodriguez-Colinas, L. Fernandez-Arrojo, A.O. Ballesteros, F.J. Plou. Galactooligosaccharides formation during enzymatic hydrolysis of lactose: towards a prebiotic enriched milk. Food Chemistry (2013). doi: 10.1016/j.foodchem.2013.08.060

Participation of the group in the SEBBM-2013 Congress

Our group has participated in the XXXVI Congress of the Spanish Society of Biochemistry and Molecular Biology (SEBBM). The work "Characterization of levansucrase from Zymomonas mobilis: levan and FOS production by soluble and immobilized enzyme" was presented in poster format by Paloma Santos.