We have published a simple and inexpensive method, based on the use of micro-centrifuge filter tubes, for establishing the best enzyme immobilization conditions. The methodology is also useful to assess the operational stability of biocatalysts.
Reference: Micro-scale procedure for enzyme immobilization screening and operational stability assays. L. Fernandez-Arrojo, P. Santos-Moriano, B. Rodriguez-Colinas, A.O. Ballesteros, F.J. Plou. Biotechnology Letters (2015), DOI 10.1007/s10529-015-1835-z
