A. Campos; L. Casas-Godoy; J.-G. Torres-Tolentino; G. Sandoval; L. Hernández; F. Plou; J. Arrizon. Lipase-catalyzed synthesis of laurate esters from puerarin and its β-D-fructofuranosyl-(2→6)-puerarin derivativeBiocatalysis and Biotransformation (2024),

J.M. Pérez de la Lastra, C.M. Curieses-Andrés, C. Andrés-Juan, F.J. Plou, E. Pérez-Lebeña. Antioxidant Metabolism Pathways in Vitamins, Polyphenols, and Selenium: Parallels and Divergences. International Journal of Molecular Sciences 25(5), 2600 (2024),

J.L. Gonzalez-Alfonso, C. Alonso, A. Poveda, Z. Ubiparip, A.O. Ballesteros, T. Desmet, J. Jiménez-Barbero, L. Coderch, and F.J. Plou. Strategy for the enzymatic acylation of the apple flavonoid phloretin based on prior α-glucosylation. Journal of Agricultural and Food Chemistry (2024),

M. Minguet-Lobato, F.V. Cervantes, N. Míguez, F.J. Plou, M. Fernández-Lobato. Chitinous material bioconversion by three new chitinases from the yeast Mestchnikowia pulcherrima. Microbial Cell Factories 23, 31 (2024)


C. Figueiredo, A. García-Ortega, T. Mandal, A. Lielpetere, F. Cervantes, D. Demurtas, E. Magner, F.J. Plou, W. Schuhmann, D. Leech, M. Pita, A.L. De Lacey. An oxygen-insensitive amperometric galactose biosensor based on galactose oxidase co-immobilized with an Os-complex modified redox polymerElectrochimica Acta 472, 143438 (2023)

L. Fernandez-Lopez, I. Cea-Rama, J. Alvarez-Malmagro, A.K. Ressmann, J.L. Gonzalez-Alfonso, C. Coscolín, P. Shahgaldian, F.J. Plou, J. Modregger, M. Pita, J. Sanz-Aparicio and M. Ferrer. Transforming an esterase into an enantioselective chatecholase through bioconjugation of a versatile metal-chelating inhibitor. Chem. Commun. 59(62), 9469-9472 (2023)

A. Robles-Martín,  R.  Amigot-Sánchez,  L.  Fernandez-Lopez,  J.L.  Gonzalez-Alfonso,  S. Roda,  V.  Alcolea-Rodriguez,  D. Heras-Márquez,  D. Almendral,  C.  Coscolín,  F.J.  Plou,  R. Portela,  M.A.  Bañares,  A. Martínez-del-Pozo,  S. García-Linares,  M.  Ferrer  &  V.  Guallar.  Sub-micro-  and  nano-sized  polyethylene  terephthalate  deconstruction  with  engineered  protein  nanopores. Nature Catalysis  (2023) 

C.M. Curieses-Andrés, J.M. Perez de Lastra, C. Andres-Juan, F. J. Plou, and E. Pérez-Lebeña. From reactive species to disease development: Effect of oxidants and antioxidants on the cellular biomarkers. Journal of Biochemical and Molecular Toxicology 37, e23455 (2023)

M.A. Distaso, T.N. Chernikova, R. Bargiela, C. Coscolín, P. Stogios, J.L. Gonzalez-Alfonso, S.  Lemak, A.N. Khusnutdinova, F.J. Plou, E. Evdokimova, A. Savchenko, E.A. Lunev, M.M. Yakimov, O.V. Golyshina, M. Ferrer, A.F. Yakunin, P.N. Golyshin. Thermophilic Carboxylesterases from Hydrothermal Vents of the Volcanic Island of Ischia Active on Synthetic and Biobased Polymers and Mycotoxins. Environmental Microbiology, 89, e01704-22 (2023)

C.M. Curieses-Andrés, J.M. Perez de Lastra, C. Andres-Juan, F.J. Plou, and E. Pérez-Lebeña. Myeloid-Derived Suppressor Cells in Cancer and COVID-19 as Associated with Oxidative Stress. Vaccines, 11, 218 (2023) doi:10.3390/vaccines11020218

M. Garcia-Gonzalez, E. Narmontaitea, F. V. Cervantes, F. J. Plou, L. Betancor, M. Fernandez-Lobato. Continuous production of honey oligosaccharides in packed-bed reactors with immobilized α-glucosidase from Metschnikowia reukaufii.  Biocatalysis and Agricultural Biotechnology, 47, 102590 (2023)  doi:10.1016/j.bcab.2022.102590

C.M. Curieses-Andrés, J.M. Perez de Lastra, C. Andres-Juan, F.J. Plou and E. Pérez-Lebeña. Superoxide Anion Chemistry—Its Role at the Core of the Innate ImmunityInternational Journal of Molecular Sciences, 24, 1841 (2023) doi:10.3390/ijms24031841


E. Jiménez-Ortega, E. Narmontaite, B. González-Pérez, F. Plou, M. Fernández-Lobato, J. Sanz-Aparicio. Insights into the structure of the highly-glycosylated Ffase from Rhodotorula dairenensis enhance its biotechnological potential. International Journal of Molecular Sciences 23(23), 14981 (2022)doi:10.3390/ijms232314981

C.M. Curieses-Andrés, J.M. Perez de Lastra, C. Andres-Juan, F.J. Plou, E. Pérez-Lebeña. Chlorine Dioxide: Friend or Foe for Cell Biomolecules? A Chemical Approach. International Journal of Molecular Sciences, 23, 15660 (2022) doi:10.3390/ijms232415660

L. Fernandez-Lopez, S. Roda, J.L. Gonzalez-Alfonso, F. J. Plou, V. Guallar, M. Ferrer. Design and Characterization of In-One Protease-Esterase PluriZyme.  International Journal of Molecular Sciences, 23, 13337 (2022) doi:10.3390/ijms232113337

M. García-González, F. V. Cervantes, R. P. Ipiales, A. de la Rubia, F. J. Plou, M. Fernández-Lobato. Isomelezitose Overproduction by Alginate-Entrapped Recombinant E. coli Cells and In Vitro Evaluation of Its Potencial Prebiotic Effect.  International Journal of Molecular Sciences, 23, 12682 (2022) doi:10.3390/ijms232012682

C. M. Curieses-Andres, J. M. Perez de la Lastra, C. Andres-Juan, F. J. Plou, E. Perez-Lebeña. The Role of Reactive Species on Innate Immunity.  Vaccines, 10, 1735 (2022) doi:10.3390/vaccines10101735

N. Miguez, D. Fernández-Polo, P. Santos-Moriano, B. Rodríguez-Colinas, A. Poveda, J. Jiménez-Barbero, A.O. Ballesteros, F.J. Plou. Enzymatic bioconversion of beechwood xylan into the antioxidant 2’-O-α-(4-O-methyl-D-glucuronosyl)-xylobiose.  Biomass Conversion and Biorefinery (2022) doi:10.1007/s13399-022-03240-3

C.M. Curieses-Andrés, J.M. Pérez de la Lastra, C. Andrés-Juan, F.J. Plou, E. Pérez-Lebeña. Hypochlorous acid chemistry in mammalian cells - Influence on infection and role in various pathologies. International Journal of Molecular Sciences 23(18), 10735 (2022) doi:10.3390/ijms231810735

S. Roda, L. Fernandez-Lopez, M. Benedens, A. Bollinger, S. Thies, J. Schumacher, C. Coscolín, M. Kazemi,Gerard Santiago,Christoph G. W. Gertzen,J.L. Gonzalez-Alfonso, F.J. Plou, K.E. Jaeger, S.H.J. Smits, M. Ferrer, V. Guallar A Plurizyme with Transaminase and Hydrolase Activity Catalyzes Cascade Reactions. Angewandte Chemie International Edition (2022) doi: 10.1002/anie.202207344

I. Cea-Rama, C. Coscolín, J.L. Gonzalez-Alfonso, J. Raj, M. Vasiljević, F.J. Plou, M. Ferrer, J. Sanz-Aparicio. Crystal structure of a family VIII β-lactamase fold hydrolase reveals the molecular mechanism for its broad substrate scope. The FEBS Journal (2022) doi: 10.1111/febs.16554

C.M. Curieses-Andrés, J.M. Perez de Lastra, C. Andres-Juan, F.J. Plou, E. Pérez-Lebeña. Impact of Reactive Species on Amino Acids—Biological Relevance in Proteins and Induced Pathologies. International Journal of Molecular Sciences,  23(22), 14049 (2022). doi:10.3390/ijms232214049

F.V. Cervantes, D. Fernandez-Polo, Z. Merdzo, N. Miguez, M. Garcia-Gonzalez, A.O. Ballesteros, M- Fernandez-Lobato and F.J. Plou. Reuse of Immobilized Komagataella phaffii Cells for the Elimination of D-Glucose in Syrups of Bioactive Carbohydrates. ACS Food Science Technology 2(4), 682–690 (2022), doi:10.1021/acsfoodscitech.2c00008 


J.L. González-Alfonso, A. Poveda, M. Arribas, Y. Hirose, M. Fernández-Lobato, A.O. Ballesteros, J. Jiménez-Barbero, and F.J. Plou. Polyglucosylation of Rutin Catalyzed by Cyclodextrin Glucanotransferase from Geobacillus sp.: Optimization and Chemical Characterization of Products. Industrial & Engineering Chemistry Research (2021), doi:10.1021/acs.iecr.1c0307

L. Fernandez-Lopez, S. Sanchez-Carrillo, A. García-Moyano, E. Borchert, D. Almendral, S. Alonso, I. Cea-Rama, N. Miguez, Ø. Larsen, J. Werner, K. S .Makarova, F. J. Plou, T. G. Dahlgren, J. Sanz-Aparicio, U. Hentschelc, G.Elin Kjæreng Bjerg and M. Ferrer. The bone-degrading enzyme machinery: from multi-component understanding to the treatment of residues from the meat industry. Computational and Structural Biotechnology Journal  19, 6328-6342 (2021), doi:10.1016/j.csbj.2021.11.027

J.A. Méndez-Líter, I. Ayuso-Fernández, F. Csarman, L.I. de Eugenio, N. Míguez, F.J. Plou ,A. Prieto , R. Ludwig and M.J. Martínez. Lytic Polysaccharide Monooxygenase from Talaromyces amestolkiae with an Enigmatic Linker-like Region: The Role of This Enzyme on Cellulose Saccharification. International Journal of Molecular Sciences  22(24), 13611 (2021), doi:10.3390/ijms222413611

J.M. Pérez de la Lastra, C. Andrés-Juan, F.J. Plou and E. Pérez-Lebeña. Theoretical Three-Dimensional Zinc Complexes with Glutathione, Amino Acids and Flavonoids. Stresses, 1(3), 123-141 (2021),  doi: 10.3390/stresses1030011

W. Snoch, D. Wnuk, T. Witko , J. Staroń , A. J. Bojarski , E. Jarek , F. J. Plou and M. Guzik. In Search of Effective Anticancer Agents—Novel Sugar Esters Based on Polyhydroxyalkanoate Monomers. International Journal of Molecular Sciences 22, 7238 (2021), doi: 10.3390/ijms22137238

J.L. González-Alfonso, Z. Ubiparip, E. Jiménez-Ortega, A. Poveda, C. Alonso, L. Coderch, J. Jimenez-Barbero, J. Sanz-Aparicio, A. Ballesteros, T. Desmet and F.J. Plou. Enzymatic Synthesis of Phloretin α‐Glucosides using a Sucrose Phosphorylase Mutant and its Effect on Solubility, Antioxidant Properties and Skin Absorption. Advanced Synthesis & Catalysis 363,  3079-3089 (2021), doi: 10.1002/adsc.202100201

C. Andrés-Juan, J.M. Pérez de la Lastra, F.J. Plou  and E. Pérez-Lebeña. The Chemistry of Reactive Oxygen Species (ROS) Revisited: Outlining Their Role in Biological Macromolecules (DNA, Lipids and Proteins) and Induced Pathologies. International Journal of Molecular Sciences, 22, 4642, (2021), doi: 10.3390/ijms22094642

N. Míguez, P. Kidibule, P. Santos-Moriano, A.O. Ballesteros, M. Fernández-Lobato and F.J. Plou. Enzymatic Synthesis and Characterization of Different Families of Chitooligosaccharides and Their Bioactive Properties.  Applied Sciences 11, 3212 (2021), doi: 10.3390/app11073212

J.M. Pérez de la Lastra, C. Andrés-Juan, F.J. Plou  and E. Pérez-Lebeña. Impact of Zinc, Glutathione, and Polyphenols as Antioxidants in the Immune Response against SARS-CoV-2. Processes, 9, 506, (2021),

P.E. Kidibule, J. Costa, A. Atrei, F.J. Plou, M. Fernández-Lobato and R. Pogni. Production and characterization of chitooligosaccharides by the fungal chitinase Chit42 immobilized on magnetic nanoparticles and chitosan beads: selectivity, specificity andimproved operational utilityRSC Advances, 11; 5529 (2021),  doi: 10.1039/d0ra10409d

D. Rodrigo-Frutos, E. Jiménez-Ortega, D. Piedrabuena, M. Ramírez-Escudero, N. Míguez, F.J. Plou, J. Sanz-Aparicio & M. Fernández-Lobato. New insights into the molecular mechanism behind mannitol and erythritol fructosylation by β-fructofuranosidase from Schwanniomyces occidentalis. Scientific Reports 11,  7158 (2021),   doi: 10.1038/s41598-021-86568-6 

A.R. Alcántara and F.J. Plou. Special Issue on “Applied Biocatalysis in Europe: A Sustainable Tool for Improving Life Quality. Catalysts, 11, 339 (2021), doi: 10.3390/catal11030339

C. V. Yáñez-Ñeco, F. V. Cervantes, L. Amaya-Delgado, A. O. Ballesteros, Francisco J. Plou, J. Arrizón. Synthesis of β(1→3) and β(1→6) galactooligosaccharides from lactose and whey using a recombinant β-galactosidase from Pantoea anthophila. Electronic Journal of Biotechnology 49; 14-21 (2021), doi: 10.1016/j.ejbt.2020.10.004


P.E. Kidibule, P. Santos-Moriano, F.J. Plou, M. Fernández-Lobato. Endo-chitinase Chit33 specificity on different chitinolytic materials allows the production of unexplored chitooligosaccharides with antioxidant activity. Biotechnology Reports, 27; e00500 (2020)doi: 10.1016/j.btre.2020.e00500

M. García-González, M. Minguet-Lobato, F. J. Plou and M. Fernández-Lobato . Molecular characterization and heterologous expression of two α-glucosidases from Metschnikowia spp, both producers of honey sugars.  Microbial Cell Factories 19; 140 (2020)doi: 10.1186/s12934-020-01397-y

F.V. Cervantes, S. Neifar, Z. Merdzo, J. Viña-González, L. Fernández-Arrojo, A.O. Ballesteros, M. Fernández-Lobato, S. Bejar and F. J. Plou. A Three-Step Process for the Bioconversion of Whey Permeate into a Glucose-Free D-Tagatose Syrup.  Catalysts, 10; 647, (2020)doi: 10.3390/catal10060647

V. Füreder, B. Rodríguez-Colinas, F.V. Cervantes, L. Fernández-Arrojo, A. Poveda, J. Jiménez-Barbero, A.O. Ballesteros and F.J. Plou. Selective Synthesis of Galactooligosaccharides Containing β(1→3) Linkages with β-Galactosidase from Bifidobacterium bifidum (Saphera).  Journal of Agricultural and Food Chemistry, 68; 4930-4938, (2020)doi: 10.1021/acs.jafc.0c00997

S. Neifar, F.V. Cervantes, H. BenHlima, A. Bouanane-Darenfed, A.O. Ballesteros, F.J. Plou and S. Bejar. Immobilization of the glucose isomerase from Caldicoprobacter algeriensis on Sepabeads EC-HA and its efficient application in continuous High Fructose Syrup production using packed bed reactor. Food Chemistry,  309;  125710, (2020)doi: 10.1016/j.foodchem.2019.125710
S. Alonso, G. Santiago, I. Cea-Rama, L. Fernández-Lopez, C. Coscolín, J. Modregger, A. K. Ressmann, M. Martínez-Martínez, H. Marrero, R. Bargiela, M. Pita, J. L. González-Alfonso, M. L. Briand, D. Rojo, C. Barbas, F. J. Plou, P. N. Golyshin, P. Shahgaldian, J. Sanz-Aparicio, V. Guallar and M. Ferrer. Genetically engineered proteins with two active sites for enhanced biocatalysis and synergistic chemo- and biocatalysis.  Nature Catalysis, 3; 319-328, (2020)doi: 10.1038/s41929-019-0394-4


M. Ramírez-Escudero, N. Míguez, M. Gimeno-Pérez, A.O. Ballesteros, M. Fernández-Lobato, F.J. Plou and J. Sanz-Aparicio. Deciphering the molecular specificity of phenolic compounds as inhibitors or glycosyl acceptors of β fructofuranosidase from Xanthophyllomyces dendrorhous.  Scientific Reports 9; 17441, (2019), doi: 10.1038/s41598-019-53948-y

M. García-González, F.V. Cervantes, F.J. Plou, M. Remacha, A. Poveda, J. Jiménez-Barbero and M. Fernández-Lobato. Efficient production of isomelezitose by a novel glucosyltransferase activity in Metschnikowia reukaufii cell extracts. Microbial Biotechnology 12, 6, 1274-1285(2019), doi: 10.1111/1751-7915.13490

H. García-Arellano, J.L. González-Alfonso, C. Ubilla, F. Comelles, M. Alcalde, M. Bernabé, J-L Parra, A.O. Ballesteros and F.J. Plou. Production and surfactant properties of tert-butyl α-D-glucopyranosides catalyzed by cyclodextrin Glucanotransferase. Catalysts, 9; 575, (2019)doi: 10.3390/catal9070575

P. Santos-Moriano, P. Kidibule, N. Míguez, L. Fernández-Arrojo, A.O. Ballesteros, M. Fernández-Lobato and F.J. Plou. Tailored enzymatic synthesis of chitooligosaccharides with different deacetylation degrees and their anti-inflammatory activity. Catalysts,  9; 405, (2019)doi: 10.3390/catal9050405

P. Gómez de Santos, F.V. Cervantes, F. Tieves, F.J. Plou, F. Hollmann, M. Alcalde. Benchmarking of laboratory evolved unspecific peroxygenases for the synthesis of human drug metabolites. Tetrahedron, 75; 1827-1831, (2019), doi: 10.1016/j.tet.2019.02.013

P. Molina-Espeja, P. Santos-Moriano, E. García-Ruiz, A. Ballesteros, FJ. Plou and M. Alcalde. Structure-Guided Immobilization of an Evolved Unspecific Peroxygenase. International Journal of Molecular Sciences, 20; 1627, (2019), doi: 10.3390/ijms20071627

J.L. González-Alfonso, P. Peñalver, A.O. Ballesteros, J.C. Morales and F.J. Plou. Effect of a-glucosylation on the stability, antioxidant properties, toxicity and neuroprotective activity of (-)-epigallocatechin gallate. Frontiers in Nutrition, 6; 30, (2019), doi: 10.3389/fnut.2019.00030


J.L. González-Alfonso, L. Casas-Godoy, J. Arrizón, D. Arrieta-Báez, A.O. Ballesteros, G. Sandoval and F.J. Plou. Lipase-catalyzed synthesis of fatty acid esters of trisaccharides. Methods Molecular Biology, 1835; 287-296. (2018), doi: 10.1007/978-1-4939-8672-9_15

J.L. González-Alfonso, N. Míguez, J.D. Padilla, L. Leemans, A. Poveda, J. Jiménez-Barbero, A.O. Ballesteros, G. Sandoval and F.J. Plou. Optimization of Regioselective Glucosylation of Hesperetin Catalyzed by Cyclodextrin Glucanotransferase. Molecules, 23; 2285, (2018), doi: 10.3390/molecules23112885

N. Míguez, M. Ramírez-Escudero, M. Gimeno-Pérez, A. Poveda, J. Jiménez-Barbero, A.O. Ballesteros, M. Fernández-Lobato, J. Sanz-Aparicio and F.J. Plou. Fructosylation of hydroxytyrosol by the β-fructofuranosidase from Xanthophyllomyces dendrorhous: Insights into the molecular basis of the enzyme specificity. ChemCatChem, 10; 4878-4887, (2018), doi:10.1002/cctc.201801171 

P. Santos-Moriano, P.E. Kidibule, E. Alleyne, A.O. Ballesteros, A. Heras, M. Fernández-Lobato and F.J. Plou. Efficient conversion of chitosan into chitooligosaccharides by a chitosanolytic activity from Bacillus thuringiensis. Process Biochemistry, 73; 102-108, (2018), doi:10.1016/j.procbio.2018.07.017

J.L. González-Alfonso, L. Leemans, A. Poveda, J. Jiménez-Barbero, A.O. Ballesteros and F.J. Plou. Efficient α-glucosylation of epigallocatechin gallate catalyzed by cyclodextrin glucanotransferase from Thermoanaerobacter sp. Journal of Agricultural and Food Chemistry 66; 7402–7408 (2018), doi:10.1021/acs.jafc.8b02143

J.L. González-Alfonso, D. Rodrigo-Frutos, E. Belmonte-Reche, P. Peñalver, A. Poveda, J. Jiménez-Barbero, A.O. Ballesteros, Y. Hirose, J. Polaina, J.C. Morales, M. Fernández-Lobato and F.J. Plou. Enzymatic synthesis of a novel pterostilbene α-glucoside by the combination of cyclodextrin glucanotransferase and amyloglucosidase. Molecules, 25; 23, (2018), doi:10.3390/molecules23061271 

N. Míguez, M. Gimeno-Pérez, D. Fernández-Polo, F.V. Cervantes, A.O. Ballesteros, M. Fernández-Lobato, M.H. Ribeiro and F.J Plou. Immobilization of the β-fructofuranosidase from Xanthophyllomyces dendrorhous by entrapment in polyvinyl alcohol and its application to neo-fructooligosaccharides production. Catalysts, 8; 201 (2018), doi:10.3390/catal8050201

B. Gómez-Fernández, E. García-Ruiz, J. Martin, P. Gómez de Santos, P. Santos-Moriano, F.J. Plou, A. Ballesteros, M. García, M. Rodríguez, V.A. Risso, J.M. Sánchez-Ruiz, S.M. Whitney and M. Alcalde. Directed -in vitro- evolution of Precambrian and extant Rubiscos. Scientific Reports, 8; 532 (2018), doi:10.1038/s41598-018-23869-3

P.E. Kidibule,  P. Santos-Moriano, E. Jiménez-Ortega, M. Ramírez-Escudero, M.C. Limón, M. Remacha, F.J. Plou, J. Sanz-Aparicio and  M. Fernández-Lobato. Use of chitin and chitosan to produce new chitooligosaccharides by chitinase Chit42: enzymatic activity and structural basis of protein specificity. Microbial Cell Factories, 17; 47 (2018),  doi: 10.1186/s12934-018-0895-x

A.R. Alcántara, E. García-Junceda, V. Gotor-Santamaría, F.J. Plou. Biocatalysis in Spain: a field of success and innovation. Biocatalysis and Biotransformation, 36; 180-183 (2018),  doi:10.1080/ 10242422.2017.1420064

P. Santos-Moriano, L. Fernández-Arrojo, M. Mengibar, E. Belmonte-Reche, P. Peñalver, F.N. Acosta, P. Kidibule, A.O. Ballesteros, J.C. Morales, M. Fernández-Lobato and F.J. Plou. Enzymatic production of fully deacetylated chitooligosaccharides and their neuroprotective and anti-inflammatory properties. Biocatalysis and Biotransformation 36; 57-67 (2018), doi: 10.1080/10242422.2017.1295231 Digital CSIC


C.V. Yáñez-Ñeco, B. Rodríguez-Colinas, L. Amaya-Delgado, A.O. Ballesteros, A.G. schaedler, F.J. Plou and J. Arrizon. Galactooligosaccharide production from Pantoea anthophila strains isolated from “Tejuino”, a Mexican traditional fermented beverage. Catalysts 7; 242 (2017), doi:10.3390/catal7080242

M. Nieto-Domínguez, L.I. de Eugenio, P. Peñalver, E. Belmonte-Reche, J.C. Morales, A. Poveda, J. Jimenez-Barbero, A. Prieto and F.J. Plou, M.J. Martínez. Enzymatic synthesis of a novel neuroprotective hydroxytyrosol glycoside. Journal of Agricultural and Food Chemistry 65; 10526-10533 (2017), doi:10.1021/acs.jafc.7b04176

L. Deladino, A. Schneider-Teixeira, F.J. Plou, A.S. Navarro and A.D. Molina-García. Effect of high hydrostatic pressure, alkaline and combined treatments on corn starch granules metal binding: structure, swelling behavior and thermal properties assessment. Food and Bioproducts Processing 102; 241-249 (2017), doi:10.1016/j.fbp.2017.01.003

M. Nieto-Domínguez, L.I. de Eugenio, M.J. York-Durán, B. Rodríguez-Colinas, F.J Plou, E. Chenoll, E. Pardo, F. Codoñer and M.J. Martínez. Prebiotic effect of xylooligosaccharides produced from birchwood xylan by a novel fungal GH11 xylanase. Food Chemistry, 232; 105-113 (2017), doi:10.1016/j.foodchem.2017.03.149


B. Rodríguez-Colinas, L. Fernández-Arrojo, P. Santos-Moriano, A.O. Ballesteros and F.J. Plou. Continuous packed bed reactor with immobilized β-galactosidase for production of galactooligosaccharides (GOS). Catalysts 6(12), 189 (2016). Special Issue "Immobilized Enzymes: Strategies for Enzyme Stabilization". doi:10.3390/catal6120189

L. Santibáñez, L. Fernández-Arrojo, C. Guerrero, F.J. Plou and A. Illanes. Removal of lactose in crude galacto-oligosaccharides by beta-galactosidase from Kluyveromyces lactis. Journal of Molecular Catalysis B: Enzymatic, 133, 85-91 (2016), doi:10.1016/j.molcatb.2016.07.014

P. Santos-Moriano, J.M. Woodley and F.J. Plou. Continuous production of chitooligosaccharides by an immobilized enzyme in a dual-reactor system. Journal of Molecular Catalysis B: Enzymatic 133, 211-217 (2016), doi:10.1016/j.molcatb.2016.09.001

P. Santos-Moriano, L. Monsalve-Ledesma, M. Ortega-Muñoz, L. Fernández-Arrojo, A.O. Ballesteros, F. Santoyo-González and F.J. Plou. Vinyl sulfone-activated silica for efficient covalent immobilization of alkaline unstable enzymes: application to levansucrase for fructooligosaccharides synthesis. RSC Advances (2016), doi: 10.1039/C6RA14046G 

D. Piedrabuena, N. Míguez, A. Poveda, F.J. Plou and M. Fernández-Lobato. Exploring the transferase activity of Ffase from Schwanniomyces occidentalis, a β-fructofuranosidase showing high fructosyl-acceptor promiscuity. Applied Microbiology and Biotechnology (2016), doi:10.1007/s00253-016-7628-z 

L. Casas-Godoy, J. Arrizón, D. Arrieta, F.J. Plou and G. Sandoval. Synthesis and emulsifying properties of carbohydrate fatty acid esters produced from Agave tequilana fructans by enzymatic acylation. Food Chemistry 204, 437-443 (2016). doi:10.1016/j.foodchem.2016.02.153 

P. Gutiérrez-Alonso, M. Gimeno-Pérez, M. Ramírez-Escudero, F.J. Plou, J. Sanz-Aparicio and M. Fernández-Lobato. Molecular characterization and heterologous expression of a Xanthophyllomyces dendrorhous α-glucosidase with potential for prebiotics productionApplied Microbiology and Biotechnology (2016) doi: 10.1007/s00253-015-7171 

P. Zambelli, L. Tamborini, S. Cazzamalli, A. Pinto, S. Arioli, S. Balzaretti, F.J. Plou, L. Fernández-Arrojo, F. Molinari, P. Conti and D. Romano. An efficient continuous flow process for the synthesis of a non-conventional mixture of fructooligosaccharides. Food Chemistry 190, 607-613 (2016) doi:10.1016/j.foodchem.2015.06.002 


P. Gutiérrez-Alonso, M. Gimeno-Pérez, M. Ramírez-Escudero, F.J. Plou, J. Sanz-Aparicio and M. Fernández-Lobato. Molecular characterization and heterologous expression of a Xanthophyllomyces dendrorhous α-glucosidase with potential for prebiotics production.  Applied Microbiology and Biotechnology (2015)doi: 10.1007/s00253-015-7171

G. Sandoval, P.G. Quintana, A. Baldessari, A.O. Ballesteros and F.J. Plou. Lipase-catalyzed preparation of mono- and diesters of ferulic acid. Biocatalysis & Biotransformation, 33, 89-97  (2015), doi:10.3109/10242422.2015.1060228

P. Santos-Moriano, L. Fernández-Arrojo, B. Rodríguez-Colinas, A.O. Ballesteros and F.J. Plou. Enzymatic synthesis of fructooligosaccharides that stimulate the gut microbiota.  Anales de la Real Academia Nacional de Farmacia  81, 48-62 (2015). Download

P. Santos-Moriano, L. Fernández-Arrojo, A. Poveda, J. Jiménez-Barbero, A.O. Ballesteros and F.J. Plou. Levan versus fructooligosaccharide synthesis using the levansucrase from Zymomonas mobilis: effect of reaction conditions. Journal of Molecular Catalysis B: Enzymatic, 119, 18–25 (2015) doi:10.1016/j.molcatb.2015.05.011 Digital CSIC

L. Fernández-Arrojo, P. Santos-Moriano, B. Rodríguez-Colinas, A.O. Ballesteros and F.J. Plou. Micro-scale procedure for enzyme immobilization screening and operational stability assays. Biotechnology Letters, 37, 1593-1600  (2015), doi:10.1007/s10529-015-1835-z Digital CSIC

P. Zambelli, I. Serra, L. Fernández-Arrojo, F.J. Plou, L. Tamborini, P. Conti, M.I. Contente, F. Molinari and D. Romano. Sweet-and-salty biocatalysis: fructooligosaccharides production using Cladosporium cladosporioides in seawater. Process Biochemistry, 50, 1086-1090 (2015), doi:10.1016/j.procbio.2015.04.006 

N. Corzo, J. L. Alonso, F. Azpiroz, M. A. Calvo, M. Cirici, R. Leis, F. Lombó, I. Mateos-Aparicio, F. J. Plou, P. Ruas-Madiedo, P. Rupérez,A. Redondo-Cuenca, M. L. Sanz and A. Clemente. Prebióticos; concepto, propiedades y efectos beneficiososNutrición Hospitalaria 31 (Supl. 1), 99-118 (2015). Download

M. Gimeno, D. Linde, L. Fernández-Arrojo, F.J. Plou and M. Fernández Lobato. Heterologous over-production of β-fructofuranosidase from yeast Xanthophyllomyces dendrorhous, an enzyme producing prebiotic sugarsApplied Microbiology and Biotechnology, 99, 3459-3467  (2015), doi:10.1007/s00253-014-6145-1


A. Kunamneni, F.J. Plou, M. Alcalde and A. Ballesteros. Trichoderma enzymes for food industries. In:  “Biotechnology and Biology of Trichoderma” (Eds. V. Gupta, M. Schmoll, A. Herrera-Estrella, R. Upadhyay, I. Druzhinina, M. Tuohy). Elsevier (2014)
ISBN 978-0-444-59576-8, pp. 339-344. 

P. Zambelli, L. Fernández-Arrojo, D. Romano, P. Santos-Moriano, M. Gimeno-Pérez, A. Poveda, R. Gandolfi, M. Fernández-Lobato, F. Molinariand and F.J. Plou. Production of fructooligosaccharides by mycelium-bound transfructosylation activity present in Cladosporium cladosporioides and Penicilium sizovae. Process Biochemistry 49, 2174-2180 (2014)  doi:10.1016/j.procbio.2014.09.021 Digital CSIC

F.J. Plou, L. Fernández-Arrojo, P. Santos-Moriano and A.O. Ballesteros. Application of immobilized enzymes for the synthesis of bioactive fructooligosaccharides. In: “Food Oligosaccharides: production, analysis and bioactivity” (Eds.  J. Moreno and M.L. Sanz). Wiley-Blackwell (2014), pp. 200-216. Digital CSIC

M. Gimeno-Pérez, P. Santos-Moriano, L. Fernández-Arrojo, A. Poveda, J. Jiménez-Barbero, A.O. Ballesteros, M. Fernández-Lobato and F.J. Plou. Regioselective synthesis of neo-erlose by the β-fructofuranosidase from Xanthophyllomyces dendrorhous. Process Biochemistry, 49, 423-429 (2014) Digital CSIC

B. Rodriguez-Colinas, L. Fernandez-Arrojo, A.O. Ballesteros, F.J. Plou. Galactooligosaccharides formation during enzymatic hydrolysis of lactose: towards a prebiotic enriched milk.  Food Chemistry, 145, 388-394 (2014), Digital CSIC 


B. Rodríguez-Colinas, L. Fernández-Arrojo, M. de Abreu, P. Urrutia, M. Fernández-Lobato, A.O. Ballesteros and F.J. Plou.“On the enzyme specificity for the synthesis of prebiotic galactooligosaccharides”. In: “Advances in Enzyme Biotechnology” (Eds.: P. Shukla and Brett Pletschke). Springer (2013), chapter 3.

T. Fontecave, C. Boissière, N. Baccile, F.J. Plou and C. Sánchez. Using evaporation induced self assembly for the direct drug templating of therapeutic vectors with high loading fractions, tunable drug release and controlled degradation. Chemistry of Materials 25, 4671–4678 (2013)doi: 10.1021/cm401807mPostprint Digital CSIC 

B. Rodríguez-Colinas, S. Kolida, M. Baran, A.O. Ballesteros, R.A. Rastall and F.J. Plou. Analysis of fermentation selectivity of purified galacto-oligosaccharides by in vitro human faecal fermentationApplied Microbiology and Biotechnology97, 5743-5752 (2013) doi:10.1007/s00253-013-4892-zPostprint Digital CSIC

S. Ferreira-Días, G. Sandoval, F. Plou and F. Valero. The potential use of lipases in the production of fatty acid derivatives for the food and nutraceutical industries. Electronic Journal of Biotechnology, Vol. 16, No.3 (2013) Digital CSIC

L. Fernández-Arrojo, B. Rodríguez-Colinas, P. Gutiérrez-Alonso, M. Fernández-Lobato, M. Alcalde, A.O. Ballesteros and F.J. Plou. Dried alginate-entrapped enzymes (DALGEEs) and their application to the production of fructooligosaccharides.Process Biochemistry,  48, 677-682 (2013). doi:10.1016/j.procbio.2013.02.015Postprint Digital CSIC

P. Urrutia, B. Rodríguez-Colinas, L. Fernández-Arrojo, A.O. Ballesteros, L. Wilson, A. Illanes and F.J. Plou Detailed analysis of galactooligosaccharides synthesis with β-galactosidase from Aspergillus oryzae. Journal of Agricultural and Food Chemistry, 61, 1081–1087 (2013). doi: 10.1021/jf304354u 


M. Álvaro-Benito, M.A. Sainz-Polo, D. González-Perez, B. González, F.J. Plou, M. Fernandez-Lobato and J. Sanz-Aparicio. Structural and kinetic insights reveal that the amino acid pair Gln-228/Asn-254 modulates the transfructosylating specificity of Schwanniomyces occidentalis β-fructofuranosidase, an enzyme that produces prebiotics. Journal of Biological Chemistry, 287, 19674-19686 (2012)

V. Barba, F.J. Plou and M.J. Martínez. Recombinant sterol esterase from Ophiostoma piceae: an improved biocatalyst expressed in Pichia pastoris.  Microbial Cell Factories, 11:73 (2012). doi:10.1186/1475-2859-11-73

D. Linde, B. Rodríguez-Colinas, M. Estévez, A. Poveda, F.J. Plou and M. Fernández-Lobato. Analysis of neofructooligosaccharides production mediated by the extracellular beta-fructofuranosidase from Xanthophyllomyces dendrorhous. Bioresource Technology, 23,123-130(2012) doi: 10.1016/j.biortech.2012.01.023 

B. Rodríguez-Colinas, A. Poveda, J. Jímenez-Barbero, A.O. Ballesteros and F.J. Plou. Galacto-oligosaccharide synthesis from lactose solution or skim milk using the β-galactosidase from Bacillus circulans. Journal of Agricultural and Food Chemistry, 60, 6391-6398 (2012). doi:10.1021/jf301156vPostprint Digital CSIC 

N.R. Aimaretti, C.V. Ybalo, M.L. Rojas, F.J. Plou andJ.C. Yori. Production of bioethanol from carrot discards. Bioresource Technology, 123, 727-732(2012). doi:10.1016/j.biortech.2012.08.035 

P. Torres, D. Reyes-Duarte, A. Ballesteros and F.J. Plou. Lipase-catalyzed modification of phenolic antioxidants. In: Lipases and Phospholipases: Methods and protocols”  (Ed. G. Sandoval). Springer Science, Methods in Molecular Biology, Vol. 861, Chapter 24, pp. 435-443 (2012).


B. Rodríguez-Colinas, M. de Abreu, L. Fernández-Arrojo, R. de Beer, A. Poveda, J. Jiménez-Barbero, D. Haltrich, A. Ballesteros, M. Fernéndez-Lobato and F.J. Plou. Production of galacto-oligosaccharides by the beta-galactosidase from Kluyveromyces lactis: comparative analysis of permeabilized cells versus soluble enzyme. Journal of Agricultural and Food Chemistry, 59, 10477-10484 (2011) doi:10.1021/jf2022012. Postprint Digital CSIC

M.A. de Abreu, M. Álvaro-Benito, F.J. Plou, M. Fernández-Lobato and M. Alcalde. Screening beta-fructofuranosidases mutant libraries to enhance the transglycosylation rates of beta-(2→6) fructooligosaccharides. Combinatorial Chemistry & High-Throughput Screening, 14, 730-738 (2011) doi:10.2174/138620711796504370 

C. Guerrero, C. Vera, F.J. Plou and A. Illanes. Influence of reaction conditions on the selectivity of the synthesis of lactulose with microbial β-galactosidases. Journal of Molecular Catalysis B: Enzymatic, 72, 206-212 (2011) doi:10.1016/j.molcatb.2011.06.007

P. Torres, A. Poveda, J. Jiménez-Barbero, J.L. Parra, F. Comelles, A. Ballesteros and F.J. Plou. Enzymatic synthesis of alpha-glucosides of resveratrol with surfactant activity. Advanced Synthesis and Catalysis, 353, 1077-1086 (2011) doi: 10.1002/adsc.201000968Postprint Digital CSIC

D. Reyes-Duarte, N. López-Cortes, P. Torres, F. Comelles, J. L. Parra, S. Peña, A. V. Ugidos, A. Ballesteros and F. J. Plou. Synthesis and properties of ascorbyl esters catalyzed by Lipozyme TL IM using triglycerides as acyl donors.  Journal of American Oil Chemists’ Society, 88, 57-64 (2011) doi: 10.1007/s11746-010-1643-5

P. Torres-Salas, A. del Monte-Martínez, B. Cutiño-Ávila, B. Rodríguez-Colinas, M. Alcalde, A. O. Ballesteros and F. J. Plou. Immobilized biocatalysts: novel approaches and tools for binding enzymes to supports. Advanced Materials, 23, 5275–5282 (2011) doi:10.1002/adma.201101821. 


I. Medina, D. Alcántara, María J. González, P. Torres, R. Lucas, J. Roque, F.J. Plou and J.C. Morales. Antioxidant Activity of Resveratrol in Several Fish Lipid Matrices: Effect of Acylation and Glucosylation. Journal of Agricultural and Food Chemistry 58 (17), 9778–9786 (2010)

F.J. Plou, P. Torres, D. Reyes-Duarte and A. Ballesteros. Enzymatic synthesis of vitamin E acetate as an alternative to the chemical method. In: "Vitamin E: Nutrition, Side Effects and Supplements". Editor: A.E. Lindberg. Nova Science Publishers, NY, USA. ISBN: 978-1-61728-757-2 (2010).

L. Gardossi, P.B. Poulsen, A. Ballesteros, K. Hult, V.K. Švedas, Đ. Vasić-Rački, G. Carrea, A. Magnusson, A. Schmid, R. Wohlgemuth and P.J. Halling. Guidelines for reporting of biocatalytic reactions. Trends in Biotechnology 28(4), 171-180 (2010). doi:10.1016/j.tibtech.2010.01.001

P. Torres, A. Poveda, J. Jiménez-Barbero, A. Ballesteros, and F.J. Plou. Regioselective Lipase-Catalyzed Synthesis of 3-O-Acyl Derivatives of Resveratrol and Study of Their Antioxidant Properties.  Journal of Agricultural and Food Chemistry, 58, 807-813 (2010). doi: 10.1021/jf903210q


D. Linde, I. Macías, L. Fernández-Arrojo, F.J. Plou, M. Fernández-Lobato. Molecular and biochemical characterization of a beta-fructofuranosidase from Xanthophyllomyces dendrorhous. Applied and Environmental Microbiology 75, 1065-1073 (2009).doi:10.1128/AEM.02061-08. 

P.Gutiérrez-Alonso, L. Fernández-Arrojo, F.J. Plou and M. Fernández-Lobato. Biochemical characterization of a beta-fructofuranosidase fromRhodotorula dairenensis with transfructosylating activity. FEMS Yeast Research 9, 768-773 (2009). doi: 10.1111/j.1567-1364.2009.00526.x.

O. Calero-Rueda, V. Barba, E. Rodríguez, F. Plou, A.T. Martínez and M.J. Martínez. Study of a sterol esterase secreted by Ophiostoma piceae: sequence, model and biochemical properties".  Biochim. Biophys. Acta 1794, 1099-1106 (2009). doi: 10.1016/j.bbapap.2009.02.012

F.J. Plou, M. Alcalde, I. Ghazi, L. Fernández-Arrojo and A. Ballesteros. Efficient fructooligosaccharide synthesis with a fructosyltransferase from Aspergillus aculeatus. In: “Modern Biocatalysis: Stereoselective and Environmentally Friendly Reactions“ (W.D. Fessner, T. Anthonsen, Eds.), Wiley Interscience, New York (2009). 


M. Álvaro-Benito , M. de Abreu, L. Fernández-Arrojo, F.J. Plou, J. Jiménez-Barbero, A.O. Ballesteros, J. Polaina and M. Fernández-Lobato . Characterization of a beta-fructofuranosidase from Schwanniomyces occidentalis with transfructosylating activity yielding the prebiotic 6-kestose. Journal of Biotechnology 132, 75-81 (2007). 

L. Fernández-Arrojo, M. Álvaro, I. Ghazi, M. de Abreu, D. Linde, P. Gutiérrez-Alonso, M. Alcalde, J. Jimenez-Barbero, A. Jiménez, A. Ballesteros,  M. Fernández-Lobato and F.J. Plou. On the transfructosylation activity and selectivity of microbial beta-fructofuranosidases for the production of prebiotics. Journal of Biotechnology, 131S, S107 (2007). 

L. Fernández-Arrojo, D. Marín, A. Gómez de Segura, D. Linde, M. Alcalde, P. Gutiérrez-Alonso, I. Ghazi, F.J. Plou, M. Fernández-Lobato and A.O. Ballesteros. Transformation of maltose into prebiotic isomaltooligosaccharides by a novel alpha-glucosidase from Xantophyllomyces dendrorhousProcess Biochemistry, 42, 1530-1536 (2007).doi:10.1016/j.procbio.2007.08.007 

I. Ghazi , L. Fernández-Arrojo , H. García-Arellano , M. Ferrer , A.O. Ballesteros and F.J. Plou. Purification and kinetic characterization of a fructosyltransferase from Aspergillus aculeatus. Journal of Biotechnology 128, 204-211 (2007).