Organization of a Course on Analytical and Semipreparative HPLC

In collaboration with the Madrid Official Chemists' College and Association, the members of the Biotransformations Laboratory have organized  for second time (the first was in 2011) an Introductory Course on Analytical and Semipreparative Liquid Chromatography HPLC. Teaching included seminars and practical sessions. The number of participants was 22, coming from different cities in Spain. The practical sessions included the separation of sweeteners in a soft drink by ionic chromatography, the analysis of a mixture of polyphenols by reverse phase HPLC, the separation of the components of a synthetic biodiesel mixture, and the scaling-up of an analytical separation of carbohydrates.

Novel method for neo-erlose synthesis published in Process Biochemistry

We have shown that the beta-fructofuranosidase from X. dendrorhous fructosylates several carbohydrates that contain glucose. In particular, the enzyme produces selectively neo-erlose from maltose. In this way novel hetero-fructooligosaccharides with food and pharmaceutical applications can be obtained. 





"Regioselective synthesis of neo-erlose by the  β-fructofuranosidase from Xanthophyllomyces dendrorhous".  M. Gimeno-Perez, P. Santos-Moriano, L. Fernández-Arrojo, A. Poveda, J. Jiménez-Barbero, A.O. Ballesteros, M. Fernández-Lobato, F.J. Plou. Process Biochemistry, accepted manuscript  available online: 6-JAN-2014, doi:10.1016/j.procbio.2013.12.018