Publications


2019

J.L. Gonzalez-Alfonso, P. Peñalver, A.O. Ballesteros, J.C. Morales and F.J. Plou. Effect of a-glucosylation on the stability, antioxidant properties, toxicity and neuroprotective activity of (-)-epigallocatechin gallate. Frontiers in Nutrition (2019), doi:10.3389/fnut.2019.00030

2018

J.L. Gonzalez-Alfonso, L. Casas-Godoy, J. Arrizon, D. Arrieta-Baez, A.O. Ballesteros, G. Sandoval and F.J. Plou. Lipase-catalyzed synthesis of fatty acid esters of trisaccharides. Methods Molecular Biology, 1835:287-296. (2018), doi:10.1007/978-1-4939-8672-9_15

J.L. González-Alfonso, N. Míguez, J. D. Padilla, L. Leemans, A. Poveda, J. Jimenez-Barbero, A. O. Ballesteros, G. Sandoval and F. J. Plou. Optimization of Regioselective Glucosylation of Hesperetin Catalyzed by Cyclodextrin Glucanotransferase. Molecules, 23, 2285, (2018) doi:10.3390/molecules23112885

N. Míguez, M. Ramírez-Escudero, M. Gimeno-Pérez, A. Poveda, J. Jiménez-Barbero, A. O. Ballesteros, M. Fernández-Lobato, J. Sanz-Aparicio and F. J. Plou. Fructosylation of hydroxytyrosol by the β-fructofuranosidase from Xanthophyllomyces dendrorhous: Insights into the molecular basis of the enzyme specificity. ChemCatChem(2018) doi:10.1002/cctc.201801171 

P. Santos-Moriano, P.E. Kidibule, E. Alleyne, A.O. Ballesteros, A. Heras, M. Fernandez-Lobato, F.J. Plou. Efficient conversion of chitosan into chitooligosaccharides by a chitosanolytic activity from Bacillus thuringiensis. Process Biochemistry,  (2018). doi:10.1016/j.procbio.2018.07.017

 J.L. Gonzalez-Alfonso, L. Leemans, A. Poveda, J. Jimenez-Barbero, A.O. Ballesteros and F.J. Plou. Efficient α-glucosylation of epigallocatechin gallate catalyzed by cyclodextrin glucanotransferase from Thermoanaerobacter sp. Journal of Agricultural and Food Chemistry 66, 7402–7408 (2018)doi:10.1021/acs.jafc.8b02143

J.L. González-Alfonso, D. Rodrigo-Frutos, E. Belmonte-Reche, P. Peñalver, A. Poveda, J. Jimenez-Barbero, A.O. Ballesteros, Y. Hirose, J. Polaina, J.C. Morales, M. Fernández-Lobato and F. J. Plou. Enzymatic synthesis of a novel pterostilbene α-glucoside by the combination of cyclodextrin glucanotransferase and amyloglucosidase. Molecules 25;23(6) (2018). doi:10.3390/molecules23061271 

N. Miguez, M. Gimeno-Perez, D. Fernandez-Polo, F.V. Cervantes, A.O. Ballesteros, M. Fernandez-Lobato, M.H. Ribeiro, F.J Plou. Immobilization of the β-fructofuranosidase from Xanthophyllomyces dendrorhous by entrapment in polyvinyl alcohol and its application to neo-fructooligosaccharides production. Catalysts 8(5), 201 (2018). doi:10.3390/catal8050201

B. Gomez-Fernandez, E. Garcia-Ruiz, J. Martin, P. Gomez de Santos, P. Santos-Moriano, F.J. Plou, A. Ballesteros, M. Garcia, M. Rodriguez, V.A. Risso, J.M. Sanchez-Ruiz, S.M. Whitney, M. Alcalde. Directed -in vitro- evolution of Precambrian and extant Rubiscos. Scientific Reports 8, 532 (2018). doi:10.1038/s41598-018-23869-3

P.E. Kidibule,  P. Santos-Moriano, E. Jiménez-Ortega, M. Ramírez-Escudero, M.C. Limón, M. Remacha, F.J. Plou, J. Sanz-Aparicio,  M. Fernández-Lobato. Use of chitin and chitosan to produce new chitooligosaccharides by chitinase Chit42: enzymatic activity and structural basis of protein specificity. Microbial Cell Factories 17:47 (2018).  doi: 10.1186/s12934-018-0895-x

A.R. Alcántara, E. García-Junceda, V. Gotor-Santamaría, F.J. Plou. Biocatalysis in Spain: a field of success and innovation. Biocatalysis and Biotransformation (2018). doi:10.1080/ 10242422.2017.1420064

P. Santos-Moriano, L. Fernandez-Arrojo, M. Mengibar, E. Belmonte-Reche, P. Peñalver, F.N. Acosta, P. Kidibule, A.O. Ballesteros, J.C. Morales, M. Fernandez-Lobato and F.J. Plou. Enzymatic production of fully deacetylated chitooligosaccharides and their neuroprotective and anti-inflammatory properties. Biocatalysis and Biotransformation 36, 57-67 (2018). doi: 10.1080/10242422.2017.1295231 Digital CSIC

2017

C.V. Yañez-Ñeco, B. Rodriguez-Colinas, L. Amaya-Delgado, A.O. Ballesteros, A.Gschaedler, F.J. Plou, J. Arrizon. Galactooligosaccharide production from Pantoea anthophila strains isolated from “Tejuino”, a Mexican traditional fermented beverage. Catalysts 7(8), 242 (2017). doi:10.3390/catal7080242

M. Nieto-Domínguez, L.I. de Eugenio, P. Peñalver, E. Belmonte-Reche, J.C. Morales, A. Poveda, J. Jimenez-Barbero, A. Prieto, F.J. Plou, M.J. Martínez. Enzymatic synthesis of a novel neuroprotective hydroxytyrosol glycoside. Journal of Agricultural and Food Chemistry 65, 10526-10533 (2017), doi:10.1021/acs.jafc.7b04176

L. Deladino, A. Schneider-Teixeira, F.J. Plou, A.S. Navarro, A.D. Molina-García. Effect of high hydrostatic pressure, alkaline and combined treatments on corn starch granules metal binding: structure, swelling behavior and thermal properties assessment. Food and Bioproducts Processing 102, 241-249 (2017). doi:10.1016/j.fbp.2017.01.003

M. Nieto-Dominguez, L.I. de Eugenio, M.J. York-Durán, B. Rodríguez-Colinas, F.J Plou, E. Chenoll, E. Pardo, F. Codoñer, M.J. Martínez. Prebiotic effect of xylooligosaccharides produced from birchwood xylan by a novel fungal GH11 xylanase. Food Chemistry (2017), doi:10.1016/j.foodchem.2017.03.149

2016

B. Rodriguez-Colinas, L. Fernandez-Arrojo, P. Santos-Moriano, A.O. Ballesteros and F.J. Plou. Continuous packed bed reactor with immobilized β-galactosidase for production of galactooligosaccharides (GOS). Catalysts 6(12), 189 (2016). Special Issue "Immobilized Enzymes: Strategies for Enzyme Stabilization". doi:10.3390/catal6120189

L. Santibáñez, L. Fernández-Arrojo, C. Guerrero, F.J. Plou, A. Illanes. Removal of lactose in crude galacto-oligosaccharides by beta-galactosidase from Kluyveromyces lactis. Journal of Molecular Catalysis B: Enzymatic, 133, 85-91 (2016), doi:10.1016/j.molcatb.2016.07.014

P. Santos-Moriano, J.M. Woodley, F.J. Plou. Continuous production of chitooligosaccharides by an immobilized enzyme in a dual-reactor system. Journal of Molecular Catalysis B: Enzymatic 133, 211-217 (2016), doi:10.1016/j.molcatb.2016.09.001

P. Santos-Moriano, L. Monsalve-Ledesma, M. Ortega-Muñoz, L. Fernandez-Arrojo, A.O. Ballesteros, F. Santoyo-González, F.J. Plou. Vinyl sulfone-activated silica for efficient covalent immobilization of alkaline unstable enzymes: application to levansucrase for fructooligosaccharides synthesis. RSC Advances (2016), doi: 10.1039/C6RA14046G 

D. Piedrabuena, N. Miguez, A. Poveda, F.J. Plou, M. Fernández-Lobato. Exploring the transferase activity of Ffase from Schwanniomyces occidentalis, a β-fructofuranosidase showing high fructosyl-acceptor promiscuity. Applied Microbiology and Biotechnology (2016), doi:10.1007/s00253-016-7628-z 

L. Casas-Godoy, J. Arrizon, D. Arrieta, F.J. Plou and G. Sandoval. Synthesis and emulsifying properties of carbohydrate fatty acid esters produced from Agave tequilana fructans by enzymatic acylation. Food Chemistry 204, 437-443 (2016). doi:10.1016/j.foodchem.2016.02.153 

P. Gutierrez-Alonso, M. Gimeno-Pérez, M. Ramírez-Escudero, F.J. Plou, J. Sanz-Aparicio, M. Fernández-Lobato. Molecular characterization and heterologous expression of a Xanthophyllomyces dendrorhous α-glucosidase with potential for prebiotics productionApplied Microbiology and Biotechnology (2016) doi: 10.1007/s00253-015-7171 

P. Zambelli, L. Tamborini, S. Cazzamalli, A. Pinto, S. Arioli, S. Balzaretti, F.J. Plou, L. Fernandez-Arrojo, F. Molinari, P. Conti, D. Romano. An efficient continuous flow process for the synthesis of a non-conventional mixture of fructooligosaccharides. Food Chemistry 190, 607-613 (2016) doi:10.1016/j.foodchem.2015.06.002 

2015

P. Gutiérrez-Alonso, M. Gimeno-Pérez, M. Ramírez-Escudero, F.J. Plou, J. Sanz-Aparicio, M. Fernández-Lobato. Molecular characterization and heterologous expression of a Xanthophyllomyces dendrorhous α-glucosidase with potential for prebiotics production.  Applied Microbiology and Biotechnology (2015)doi: 10.1007/s00253-015-7171

G. Sandoval, P.G. Quintana, A. Baldessari, A.O. Ballesteros, F.J. Plou. Lipase-catalyzed preparation of mono- and diesters of ferulic acid. Biocatalysis & Biotransformation, 33, 89-97  (2015), doi:10.3109/10242422.2015.1060228

P. Santos-Moriano, L. Fernández-Arrojo, B. Rodríguez-Colinas, A.O. Ballesteros, F.J. Plou. Enzymatic synthesis of fructooligosaccharides that stimulate the gut microbiota.  Anales de la Real Academia Nacional de Farmacia  81, 48-62 (2015). Download

P. Santos-Moriano, L. Fernandez-Arrojo, A. Poveda, J. Jimenez-Barbero, A.O. Ballesteros, F.J. Plou. Levan versus fructooligosaccharide synthesis using the levansucrase from Zymomonas mobilis: effect of reaction conditions. Journal of Molecular Catalysis B: Enzymatic, 119, 18–25 (2015) doi:10.1016/j.molcatb.2015.05.011 Digital CSIC

L. Fernandez-Arrojo, P. Santos-Moriano, B. Rodriguez-Colinas, A.O. Ballesteros and F.J. Plou. Micro-scale procedure for enzyme immobilization screening and operational stability assays. Biotechnology Letters, 37, 1593-1600  (2015), doi:10.1007/s10529-015-1835-z Digital CSIC

P. Zambelli, I. Serra, L. Fernandez-Arrojo, F.J. Plou, L. Tamborini, P. Conti, M.I. Contente, F. Molinari, D. Romano. Sweet-and-salty biocatalysis: fructooligosaccharides production using Cladosporium cladosporioides in seawater. Process Biochemistry, 50, 1086-1090 (2015), doi:10.1016/j.procbio.2015.04.006 

N. Corzo, J. L. Alonso, F. Azpiroz, M. A. Calvo, M. Cirici, R. Leis, F. Lombó, I. Mateos-Aparicio, F. J. Plou, P. Ruas-Madiedo, P. Ruperez, A. Redondo-Cuenca, M. L. Sanz and A. Clemente. Prebióticos; concepto, propiedades y efectos beneficiososNutrición Hospitalaria 31 (Supl. 1), 99-118 (2015). Download

M. Gimeno, D. Linde, L. Fernández-Arrojo, F.J. Plou, M. Fernández Lobato. Heterologous over-production of β-fructofuranosidase from yeast Xanthophyllomyces dendrorhous, an enzyme producing prebiotic sugarsApplied Microbiology and Biotechnology, 99, 3459-3467  (2015), doi:10.1007/s00253-014-6145-1

2014

A. Kunamneni, F.J. Plou, M. Alcalde, A. Ballesteros. Trichoderma enzymes for food industries. In:  “Biotechnology and Biology of Trichoderma” (Eds. V. Gupta, M. Schmoll, A. Herrera-Estrella, R. Upadhyay, I. Druzhinina, M. Tuohy). Elsevier (2014)
ISBN 978-0-444-59576-8, pp. 339-344. 

P. Zambelli, L. Fernandez-Arrojo, D. Romano, P. Santos-Moriano, M. Gimeno-Perez, A. Poveda, R. Gandolfi, M. Fernández-Lobato, F. Molinari, F.J. Plou. Production of fructooligosaccharides by mycelium-bound transfructosylation activity present in Cladosporium cladosporioides and Penicilium sizovae. Process Biochemistry 49, 2174-2180 (2014)  doi:10.1016/j.procbio.2014.09.021 Digital CSIC

F.J. Plou, L. Fernandez-Arrojo, P. Santos-Moriano and A.O. Ballesteros. Application of immobilized enzymes for the synthesis of bioactive fructooligosaccharides. In: “Food Oligosaccharides: production, analysis and bioactivity” (Eds.  J. Moreno and M.L. Sanz). Wiley-Blackwell (2014), pp. 200-216. Digital CSIC

M. Gimeno-Perez, P. Santos-Moriano, L. Fernández-Arrojo, A. Poveda, J. Jiménez-Barbero, A.O. Ballesteros, M. Fernández-Lobato, F.J. Plou. Regioselective synthesis of neo-erlose by the β-fructofuranosidase from Xanthophyllomyces dendrorhous. Process Biochemistry, 49, 423-429 (2014) http://dx.doi.org/10.1016/j.procbio.2013.12.018Postprint Digital CSIC

B. Rodriguez-Colinas, L. Fernandez-Arrojo, A.O. Ballesteros, F.J. Plou. Galactooligosaccharides formation during enzymatic hydrolysis of lactose: towards a prebiotic enriched milk.  Food Chemistry, 145, 388-394 (2014), http://dx.doi.org/10.1016/j.foodchem.2013.08.060Postprint Digital CSIC 

2013



B. Rodriguez-Colinas, L. Fernandez-Arrojo, M. de Abreu, P. Urrutia, M. Fernandez-Lobato, A.O. Ballesteros and F.J. Plou.“On the enzyme specificity for the synthesis of prebiotic galactooligosaccharides”. In: “Advances in Enzyme Biotechnology” (Eds.: P. Shukla and Brett Pletschke). Springer (2013), chapter 3.

T. Fontecave, C. Boissière, N. Baccile, F.J. Plou, C. Sanchez. Using evaporation induced self assembly for the direct drug templating of therapeutic vectors with high loading fractions, tunable drug release and controlled degradation. Chemistry of Materials 25, 4671–4678 (2013)doi: 10.1021/cm401807mPostprint Digital CSIC 

B. Rodriguez-Colinas, S. Kolida, M. Baran, A.O. Ballesteros, R.A. Rastall, F.J. Plou. Analysis of fermentation selectivity of purified galacto-oligosaccharides by in vitro human faecal fermentationApplied Microbiology and Biotechnology97, 5743-5752 (2013) doi:10.1007/s00253-013-4892-zPostprint Digital CSIC

S. Ferreira-Dias, G. Sandoval, F. Plou, F. Valero. The potential use of lipases in the production of fatty acid derivatives for the food and nutraceutical industries. Electronic Journal of Biotechnology, Vol. 16, No.3 (2013) http://dx.doi.org/10.2225/vol16-issue3-fulltext-5Postprint Digital CSIC

L. Fernandez-Arrojo, B. Rodriguez-Colinas, P. Gutierrez-Alonso, M. Fernandez-Lobato, M. Alcalde, A.O. Ballesteros and F.J. Plou. Dried alginate-entrapped enzymes (DALGEEs) and their application to the production of fructooligosaccharides.Process Biochemistry,  48, 677-682 (2013). doi:10.1016/j.procbio.2013.02.015Postprint Digital CSIC

P. Urrutia, B. Rodriguez-Colinas, L. Fernandez-Arrojo, A.O. Ballesteros, L. Wilson, A. Illanes and F.J. Plou Detailed analysis of galactooligosaccharides synthesis with β-galactosidase from Aspergillus oryzae. Journal of Agricultural and Food Chemistry, 61, 1081–1087 (2013). doi: 10.1021/jf304354u 

2012



M. Alvaro-Benito, M.A. Sainz-Polo, D. Gonzalez-Perez, B. Gonzalez, F.J. Plou, M. Fernandez-Lobato, J. Sanz-Aparicio. Structural and kinetic insights reveal that the amino acid pair Gln-228/Asn-254 modulates the transfructosylating specificity of Schwanniomyces occidentalis β-fructofuranosidase, an enzyme that produces prebiotics. Journal of Biological Chemistry, 287, 19674-19686 (2012)http://dx.doi.org/10.1074/jbc.M112.355503

V. Barba, F.J. Plou and Maria J. Martinez. Recombinant sterol esterase from Ophiostoma piceae: an improved biocatalyst expressed in Pichia pastoris.  Microbial Cell Factories, 11:73 (2012). doi:10.1186/1475-2859-11-73

D. Linde, B. Rodriguez-Colinas, M. Estevez, A. Poveda, F.J. Plou and M. Fernandez-Lobato. Analysis of neofructooligosaccharides production mediated by the extracellular beta-fructofuranosidase from Xanthophyllomyces dendrorhous. Bioresource Technology, 23,123-130(2012) doi: 10.1016/j.biortech.2012.01.023 

B. Rodriguez-Colinas, A. Poveda, J. Jimenez-Barbero, A.O. Ballesteros, F.J. Plou. Galacto-oligosaccharide synthesis from lactose solution or skim milk using the β-galactosidase from Bacillus circulans. Journal of Agricultural and Food Chemistry, 60, 6391-6398 (2012). doi:10.1021/jf301156vPostprint Digital CSIC 

N.R. Aimaretti, C.V. Ybalo, M.L. Rojas, F.J. Plou, J.C. Yori. Production of bioethanol from carrot discards. Bioresource Technology, 123, 727-732(2012). doi:10.1016/j.biortech.2012.08.035 


P. Torres, D. Reyes-Duarte, A. Ballesteros and F.J. Plou. Lipase-catalyzed modification of phenolic antioxidants. In: Lipases and Phospholipases: Methods and protocols”  (Ed. G. Sandoval). Springer Science, Methods in Molecular Biology, Vol. 861, Chapter 24, pp. 435-443 (2012).

2011



B. Rodriguez-Colinas, M. de Abreu, L. Fernandez-Arrojo, R. de Beer, A. Poveda, J. Jimenez-Barbero, D. Haltrich, A. Ballesteros, M. Fernandez-Lobato and F.J. Plou. Production of galacto-oligosaccharides by the beta-galactosidase from Kluyveromyces lactis: comparative analysis of permeabilized cells versus soluble enzyme. Journal of Agricultural and Food Chemistry, 59, 10477-10484 (2011) doi:10.1021/jf2022012. Postprint Digital CSIC

M.A. de Abreu, M. Álvaro-Benito, F.J. Plou, M. Fernández-Lobato and M. Alcalde. Screening beta-fructofuranosidases mutant libraries to enhance the transglycosylation rates of beta-(2→6) fructooligosaccharides. Combinatorial Chemistry & High-Throughput Screening, 14, 730-738 (2011) doi:10.2174/138620711796504370 

C. Guerrero, C. Vera, F. Plou and A. Illanes. Influence of reaction conditions on the selectivity of the synthesis of lactulose with microbial β-galactosidases. Journal of Molecular Catalysis B: Enzymatic, 72, 206-212 (2011) doi:10.1016/j.molcatb.2011.06.007

P. Torres, A. Poveda, J. Jiménez-Barbero, J.L. Parra, F. Comelles, A. Ballesteros and F.J. Plou. Enzymatic synthesis of alpha-glucosides of resveratrol with surfactant activity. Advanced Synthesis and Catalysis, 353, 1077-1086 (2011) doi: 10.1002/adsc.201000968Postprint Digital CSIC

D. Reyes-Duarte, N. Lopez-Cortes, P. Torres, F. Comelles, J. L. Parra, S. Peña, A. V. Ugidos, A. Ballesteros and F. J. Plou. Synthesis and properties of ascorbyl esters catalyzed by Lipozyme TL IM using triglycerides as acyl donors.  Journal of American Oil Chemists’ Society, 88, 57-64 (2011) doi: 10.1007/s11746-010-1643-5

P. Torres-Salas, A. del Monte-Martinez, B. Cutiño-Avila, B. Rodriguez-Colinas, M. Alcalde, A. O. Ballesteros, F. J. Plou. Immobilized biocatalysts: novel approaches and tools for binding enzymes to supports. Advanced Materials, 23, 5275–5282 (2011) doi:10.1002/adma.201101821. 

2010



I. Medina, D. Alcántara, María J. González, P. Torres, R. Lucas, J. Roque, F.J. Plou and J.C. Morales. Antioxidant Activity of Resveratrol in Several Fish Lipid Matrices: Effect of Acylation and Glucosylation. Journal of Agricultural and Food Chemistry 58 (17), 9778–9786 (2010)

F.J. Plou, P. Torres, D. Reyes-Duarte and A. Ballesteros. Enzymatic synthesis of vitamin E acetate as an alternative to the chemical method. In: "Vitamin E: Nutrition, Side Effects and Supplements". Editor: A.E. Lindberg. Nova Science Publishers, NY, USA. ISBN: 978-1-61728-757-2 (2010).

Lucia Gardossi, Poul B. Poulsen, Antonio Ballesteros, Karl Hult, Vytas K. Švedas, Đurđa Vasić-Rački, Giacomo Carrea, Anders Magnusson, Andreas Schmid, Roland Wohlgemuth and Peter J. Halling. Guidelines for reporting of biocatalytic reactions. Trends in Biotechnology 28(4), 171-180 (2010). doi:10.1016/j.tibtech.2010.01.001

P. Torres, A. Poveda, J. Jimenez-Barbero, A. Ballesteros, and F.J. Plou. Regioselective Lipase-Catalyzed Synthesis of 3-O-Acyl Derivatives of Resveratrol and Study of Their Antioxidant Properties.  Journal of Agricultural and Food Chemistry, 58, 807-813 (2010). doi: 10.1021/jf903210q

2009



Linde D, Macias I, Fernandez-Arrojo L, Plou FJ, Fernandez-Lobato M.Molecular and biochemical characterization of a beta-fructofuranosidase from Xanthophyllomyces dendrorhous. Applied and Environmental Microbiology 75, 1065-1073 (2009).doi:10.1128/AEM.02061-08. 

Gutierrez-Alonso P, Fernandez-Arrojo L, Plou FJ, Fernandez-Lobato M.Biochemical characterization of a beta-fructofuranosidase fromRhodotorula dairenensis with transfructosylating activity. FEMS Yeast Research 9, 768-773 (2009). doi: 10.1111/j.1567-1364.2009.00526.x.

O. Calero-Rueda, V. Barba, E. Rodríguez, F. Plou, A.T. Martínez and M.J. Martínez. Study of a sterol esterase secreted by Ophiostoma piceae: sequence, model and biochemical properties".  Biochim. Biophys. Acta 1794, 1099-1106 (2009). doi: 10.1016/j.bbapap.2009.02.012

F.J. Plou, M. Alcalde, I. Ghazi, L. Fernández-Arrojo and A. Ballesteros.“Efficient fructooligosaccharide synthesis with a fructosyltransferase from Aspergillus aculeatus”. In: “Modern Biocatalysis: Stereoselective and Environmentally Friendly Reactions“ (W.D. Fessner, T. Anthonsen, Eds.), Wiley Interscience, New York (2009). 

2007



Alvaro-Benito M, de Abreu M, Fernandez-Arrojo L, Plou FJ, Jimenez-Barbero J, Ballesteros A, Polaina J, Fernandez-Lobato M. Characterization of a beta-fructofuranosidase from Schwanniomyces occidentalis with transfructosylating activity yielding the prebiotic 6-kestose. Journal of Biotechnology 132, 75-81 (2007). 

L. Fernández-Arrojo, M. Alvaro, I. Ghazi, M. de Abreu, D. Linde, P. Gutiérrez-Alonso, M. Alcalde, J. Jimenez-Barbero, A. Jimenez, A. Ballesteros,  M. Fernández-Lobato and F.J. Plou.On the transfructosylation activity and selectivity of microbial beta-fructofuranosidases for the production of prebiotics. Journal of Biotechnology, 131S, S107 (2007). 

L. Fernández-Arrojo, D. Marín, A. Gómez de Segura, D. Linde, M. Alcalde, P. Gutiérrez-Alonso, I. Ghazi, F.J. Plou, M. Fernández-Lobato and A. Ballesteros.“Transformation of maltose into prebiotic isomaltooligosaccharides by a novel alpha-glucosidase from Xantophyllomyces dendrorhousProcess Biochemistry, 42, 1530-1536 (2007).doi:10.1016/j.procbio.2007.08.007 

Ghazi I, Fernandez-Arrojo L, Garcia-Arellano H, Ferrer M, Ballesteros A, Plou FJ. Purification and kinetic characterization of a fructosyltransferase from Aspergillus aculeatus. Journal of Biotechnology 128, 204-211 (2007).