14 Jul 2017

Paloma Santos-Moriano defends her Thesis

Paloma Santos-Moriano defended her Thesis by the Autonomous University of Madrid (UAM) entitled "Biocatalytic processes for the production of bioactive carbohydrates: fructooligosaccharides and chitooligosaccharides", supervised by Dr. Francisco J. Plou. She obtained the maximum qualification (Sobresaliente cum laude). The jury was formed by Dr. Jose L. Copa-Patiño (University of Alcala), Dr. Javier Moreno (CIAL, UAM-CSIC), Dr. Beatriz Gullon (University of Santiago de Compostela), Dr. Pamela Torres (Repsol) and Dr. Miguel Remacha (UAM). CONGRATULATIONS!

7 Jul 2017

Dr. Yoshihiko Hirose visits the Applied Biocatalysis Group

Dr. Yoshihiko Hirose, enzyme application consultant at Enzyme Techno (Japan), visited our laboratories. We have established a collaboration in the field of enzymatic glycosylation of bioactive polyphenols.

29 Jun 2017

Meeting of the Spanish Society of Catalysis (SECAT 2017)

Our PhD student Jose L. González-Alfonso delivered an oral presentation during the celebration of the Meeting of the Spanish Society of Catalysis (SECAT 2017, "Catalysis for a more sustainable world") held in Oviedo (26-28 June 2017). The talk was entitled "Enzymatic glycosylation of two bioactive polyphenols: hydroxityrosol and epigallocatechin gallate", by Noa Míguez, María Gimeno-Pérez, José Luis González-Alfonso, Mercedes Ramírez-Escudero, Laura Leemans, Antonio O. Ballesteros, Julia Sanz-Aparicio, María Fernández-Lobato and Francisco J. Plou.

22 Jun 2017

Participation in the Spanish Congress of Biotechnology (BIOTEC 2017)

The Applied Biocatalysis Group at CSIC has contributed to the Spanish Congress of Biotechnology (BIOTEC 2017) held in Murcia (18-21 June 2017) with one oral presentation (by Paloma Santos-Moriano) and three poster presentations (by Jose L. Gonzalez-Alfonso, Noa Miguez and Fadia Cervantes). We have presented our latest advances in the synthesis of prebiotic oligosachharides, novel carbohydrates and glycosides of plant polyphenols.

1 Apr 2017

Production of chitooligosaccharides and their bioactive properties

Several commercial enzymes were screened for chitosanolytic activity. The hydrolysis of different chitosans was followed by size exclusion chromatography (SEC-ELSD), mass spectrometry (ESI-Q-TOF), and high-performance anion-exchange chromatography with pulsed amperometric detection (HPAEC-PAD). Neutrase 0.8L converted 10 g/L of various chitosans into mostly deacetylated oligosaccharides, yielding approximately 2.5 g/L of chitobiose, 4.5 g/L of chitotriose and 3 g/L of chitotetraose. In collaboration with the Institute of Parasitology and Biomedicine "Lopez-Neyra" (CSIC), the synthesized COS were tested in vitro for their neuroprotective and anti-inflammatory activities, and compared with other functional ingredients, namely fructooligosaccharides.

Reference: “Enzymatic production of fully deacetylated chitooligosaccharides and their neuroprotective and anti-inflammatory properties”. P. Santos-Moriano, L. Fernandez-Arrojo, M. Mengibar, E. Belmonte-Reche, P. Peñalver, F.N. Acosta, P. Kidibule, A.O. Ballesteros, J.C. Morales, M. Fernandez-Lobato and F.J. Plou. Biocatalysis and Biotransformation (2017), doi: 10.1080/10242422.2017.1295231

10 Mar 2017

High school students from Cordoba (Spain) visit the Applied Biocatalysis Group

A group of students from the High School IES-Averroes (Cordoba, Spain) have visited the Applied Biocatalysis Group at the Institute of Catalysis and Petrochemistry (CSIC). They have carried out a series of experiments related with the applications of enzymes in the food industry.

10 Oct 2016

XVIII LIGNOCEL Meeting

The XVIII meeting of the LIGNOCEL Network (Biotechnology of Lignocellulolosic Materials),  took place in Jaen (October 6-7 2016), Our group presented the work “Lipase-catalyzed preparation of mono- and diesters of ferulic acid”. The meeting was very fruitful to establish new collaborations between research groups involved in the exploitation of lignocellulosic residues.


27 Sep 2016

Participation in the VI Congress of Industrial Microbiology and Microbial Biotechnology

The Applied Biocatalysis Group has participated in the VI Congress of Industrial Microbiology and Microbial Biotechnology (CMIBM’16) held at the University of Leon, Spain (September 12-14, 2016). The Congress was auspiced by the Spanish Society of Microbiology. Our group presented an abstract entitled "Production of galactooligosaccharides using Kluyveromyces lactis permeabilized cells: effect of entrapment in alginate"

23 Sep 2016

Dual reactor for production of chitosan oligosaccharides


Chitooligosaccharides (COS), obtained by chemical or enzymatic hydrolysis of chitosan, are more soluble and exhibit enhanced biological activities compared with the polysaccharide. COS show antimicrobial, antioxidant, antiangiogenic, antitumoral, anti-inflammatory and prebiotic properties, among others. In this work, a chitosanolytic enzyme was covalently immobilized onto glyoxal agarose beads  and assessed for the continuous production of COS. A two-step process was proposed, in which chitosan was first hydrolyzed in a batch reactor to a viscosity that could flow through a packed-bead reactor (PBR), thus avoiding clogging of the column. This work was performed in collaboration with the Technical University of Denmark

Reference: “Continuous production of chitooligosaccharides by an immobilized enzyme in a dual-reactor system”. P. Santos-Moriano, J.M. Woodley, F.J. Plou. Journal of Molecular Catalysis B: Enzymatic 133, 211-217 (2016), doi:10.1016/j.molcatb.2016.09.001