9 Oct 2020
Francisco J. Plou will participate in the online seminars on "Science and Engineering applied to agrofood and biosystems" organized by University of Valladolid. He will deliver the speech "Transformation of residues rich in carbohydrates into high added-value products using enzymes" (14 October 2020, 16 h).
10 Jun 2020
We have developed a sustainable three-stage process for the bioconversion of cheese whey permeate into D-tagatose, a rare sugar with functional properties used as sweetener. The experimental conditions (pH, temperature, cofactors, etc.) for each step were independently optimized. The proposed integrated process takes place under mild conditions (neutral pH, moderate temperatures) in a short time (13 h), yielding a glucose-free syrup containing D-tagatose and galactose in a ratio 1:2 (w/w).Ref: “A three-step process for the bioconversion of whey permeate into D-tagatose syrup free of glucose”. F.V. Cervantes, S. Neifar, Z. Merdzo, J. Viña-Gonzalez, L. Fernandez-Arrojo, A.O. Ballesteros, M. Fernandez-Lobato, S. Bejar and F.J. Plou. Catalysts 10(6), 647 (2020) https://doi.org/10.3390/catal10060647
25 Feb 2020
3 Dec 2019
Reference: “Deciphering the molecular specificity of phenolic compounds as inhibitors or glycosyl acceptors of β fructofuranosidase from Xanthophyllomyces dendrorhous”. M. Ramirez-Escudero, N. Miguez, M. Gimeno-Perez, A.O. Ballesteros, M. Fernandez-Lobato, F.J. Plou* and J. Sanz-Aparicio* Scientific Reports 9, 17441 (2019), doi:10.1038/s41598-019-53948-y
22 Nov 2019
14 Nov 2019
13 Nov 2019
29 Oct 2019
We have published an article in collaboration with University of Sfax (Tunisia) and University of Sciences and Technology of Houari Boumediene (Algeria). The glucose isomerase (GICA) from Caldicoprobacter algeriensis was immobilized by ionic adsorption on polymethacrylate carriers. The Sepabeads EC-HA yielded the highest recovery of activity (89%). The adsorbed enzyme displayed higher relative activity at acidic pH, greater thermostability, and better storage stability, compared to the free form. Moreover, the immobilized enzyme showed an excellent operational stability, in 15 successive 3h reaction cycles at 85°C under a batch reactor, preserving 83% of its initial activity. Interestingly, a continuous process for High Fructose Syrup (HFS) production was established with the adsorbed GICA using a packed bed reactor during eleven days at 70°C. HPAEC-PAD analysis showed a maximum bioconversion rate of 49% after 48h of operation.
Reference: "Immobilization of the glucose isomerase from Caldicoprobacter algeriensis on Sepabeads EC-HA and its efficient application in continuous High Fructose Syrup production using packed bed reactor". S. Neifar, F.V. Cervantes, H. BenHlima, A. Bouanane-Darenfed, A.O. Ballesteros, F.J. Plou, S. Bejar. . Food Chemistry (2019), doi: 10.1016/j.foodchem.2019.125710
25 Oct 2019
2 Jul 2019
Production and Surfactant Properties of Tert-Butyl α-D-Glucopyranosides Catalyzed by Cyclodextrin Glucanotransferase
1 Jul 2019
The awarded work was: ''Enzymatic synthesis of a novel pterostilbene α-glucoside by the combination of cyclodextrin glucanotransferase and amyloglucosidase''. J.L. González-Alfonso, D. Rodrigo-Frutos, E. Belmonte-Reche, P. Peñalver, A. Poveda, J. Jimenez-Barbero, A.O. Ballesteros, Y. Hirose, J. Polaina, J.C. Morales, M. Fernández-Lobato and F. J. Plou. Molecules 25;23(6) (2018). doi:10.3390/molecules23061271
27 Jun 2019
This work was the result of a collaboration between the Laboratory of Microbial Biotechnology and Engineering Enzymes (Centre of Biotechnology of Sfax, University of Sfax, Tunisia), the Laboratory of Cellular and Molecular Biology (Faculty of Biological Sciences, University of Sciences and Technology of Houari Boumediene, Algeria) and the Instituto de Catálisis y Petroleoquímica (CSIC, Madrid, Spain).
28 Mar 2019
Francisco J. Plou has recently participated in the Second Mediterranean Congress of Biotechnology (MCB2, http://www.mcb2019.com), held from 16 to 20 March 2019 in Hammamet (Tunisia), as representative of Biocatalysis in the Spanish Society of Biotechnology (SEBIOT). Francisco Plou pronounced a conference on “Novel trends in the enzymatic transformation of carbohydrates into value-added products for the food and nutraceutical industries”.
19 Mar 2019
Article on the effect of α-glucosylation of epigallocatechin gallate published in Frontiers in Nutrition
''Effect of a-glucosylation on the stability, antioxidant properties, toxicity and neuroprotective activity of (-)-epigallocatechin gallate''. Jose L. Gonzalez-Alfonso, P. Peñalver, A.O. Ballesteros, J.C. Morales and F.J. Plou. Frontiers in Nutrition, (2019) doi: 10.3389/fnut.2019.00030
18 Feb 2019
21 Jan 2019
20 Jan 2019
The ABG, present in a Congress and a Practical Workshop on "Enzymes in the Food Industry" (ENZAL 2019, Santiago de Chile)
Our group has participated in a Practical Workshop and a Congress on "Enzymes in the Food Industry" (ENZAL 2019) held in Santiago de Chile, from January 8th to 11th 2019. Francisco Plou supervised a practical class on elimination of lactose with enzymes. Furthermore, he delivered a conference on "Enzymatic synthesis of bioactive oligosaccharides: production of chitooligosaccharides".
13 Dec 2018
28 Nov 2018
26 Nov 2018
The Applied Biocatalysis Group has participated in the "One Day Symposium: Research in Carbohydrates" organized by the Group of Carbohydrates at the Spanish Royal Society of Chemistry. The Symposium took place at the Faculty of Chemistry of University of Sevilla. The title of the conference delivered by Francisco Plou was “Recent examples of the enzymatic synthesis of bioactive oligosaccharides and polyphenol glycosides”